Figure 3: Selectivity of Mon1–Ccz1 for Ypt7 over Vps21.

(a) For CtVps21, CtYpt7 and different mutations, nucleotide exchange rates are plotted as a function of CtMC1core concentration. Error bars represent s.d. of three independent biological repeats. (b) MC1-interacting interface of Ypt7 is shown in blue. Positions that are conserved in the Ypt7 family, but not the Vps21 family, are highlighted. Labels show the mutations from Ypt7- to Vps21-specific residues. Colours correspond to the graphs in b.