Figure 4: Interaction of Mon1–Ccz1 with Ypt7. | Nature Communications

Figure 4: Interaction of Mon1–Ccz1 with Ypt7.

From: Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1–Ccz1

Figure 4

(a) Surface representation of the CtMC1 longin heterodimer. Mutations introduced in the interaction interface with Ypt7 are labelled. (b) Nucleotide exchange rates of Ypt7 are plotted as a function of the concentration of CtMC1core wild-type and different mutations. Error bars represent s.d. of three independent biological repeats. Colours of the graphs correspond to highlighted mutations in a. Functionality test of mutations in (c) mon1Δ and (d) ccz1Δ yeast knockout strains. The vacuolar fragmentation phenotype is rescued by the introduction of ScMon1 and ScCcz1, respectively, but not the mutations corresponding to the GEF-deficient mutants described above. Scale bars: 5 μm.

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