Table 1 Crystallographic statistics of the CtMC1core–Ypt7 complexes.
Ct MC1core–Ypt7 (SAD peak) | Ct MC1core–Ypt7 (pdb code: 5LDD) | |
|---|---|---|
Data collection | ||
X-ray source | Beamline P13, EMBL, Hamburg, Germany | |
Detector | PILATUS 6M | |
Wavelength (Å) | 0.97958 | 0.97951 |
Space group | P212121 | P212121 |
Cell dimensions (a,b,c Å) | 62.56, 100.57, 207.10 | 70.08, 103.81, 206.67 |
Resolution (Å) | 48.86-2.89 (3.07-2.89) | 46.38-2.49 (2.65-2.49) |
Total reflections | 392922 | 346702 |
Multiplicity | 6.97 | 6.53 |
Unique reflections | 56335 | 53101 |
Completeness (%) | 99.7 (98.6) | 99.5 (98.1) |
Rmeas (%) | 13.1 (78.5) | 7.6 (90.9) |
CC 1/2 (ref. 50) | 99.7 (84.8) | 99.9 (91.9) |
I/σ(I) | 11.77 (2.72) | 15.24 (2.62) |
Mosaicity (°) | 0.153 | 0.134 |
Wilson B-factor (Å2) | 60.89 | 69.32/46.3* |
Refinement | ||
Resolution (Å) | 46.38-2.50 | |
Rwork, Rfree | 0.200, 0.241 | |
Reflections (working, test set) | 46240, 1839 | |
Completeness for range (%) | 86.7 | |
r.m.s.d. from ideal | ||
Bond lenghts (Å) | 0.004 | |
Bond angles (°) | 0.620 | |
Total number of atoms | 7670 | |
Mean B value (Å2) | 71.3 | |
