Figure 5: TKs (a CD8+ T cell line) upregulate NCC in mDCTs via direct cell-cell contact. | Nature Communications

Figure 5: TKs (a CD8+ T cell line) upregulate NCC in mDCTs via direct cell-cell contact.

From: CD8+ T cells stimulate Na-Cl co-transporter NCC in distal convoluted tubules leading to salt-sensitive hypertension

Figure 5

(a) Image of TK cells adherent to mDCTs. Scale, 40 μm. After overnight co-culture, cells were washed with PBS × 2. Data are representative of n=10 images in each group. (b) Orthogonal section of the 3D image (XZ, XY, YZ) of individual cell-cell contact of TK (CD8, red) and mDCT (Na-K-ATPase, NKA, green) cells in co-culture for 4 h. Scale, 2 μm. Data are representative of n>15 images. All images were captured and processed with super-resolution 3D-SIM microscopy. (c) Total protein expression of NCC and p-NCC in mDCTs with or without TK co-culture. GAPDH was used as a loading control. n=4–6 in each group. Data are means±s.e. *P<0.01 versus Control (t-test) (d) Upper, effects of 0.4 and 8 μm transwells on TK-mediated up-regulation of NCC and p-NCC in mDCT membrane protein. Lower, effects of transwells on TK-mediated up-regulation of NCC and full length SPAK expression in mDCT total lysate. All groups with or without transwells used the same culture conditions. Na-K-ATPase or GAPDH were used as loading controls of membrane protein or total protein, respectively. n=4 in each group. Data are means±s.e. *P<0.01 versus Control (ANOVA).

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