Figure 8: Effects of ClC-K or Kir4.1 knockdown on TK-mediated NCC-regulating pathway in mDCTs. | Nature Communications

Figure 8: Effects of ClC-K or Kir4.1 knockdown on TK-mediated NCC-regulating pathway in mDCTs.

From: CD8+ T cells stimulate Na-Cl co-transporter NCC in distal convoluted tubules leading to salt-sensitive hypertension

Figure 8

(a) In membrane proteins (upper), ClC-K knockdown prevented TK-induced membrane expression of p-NCC and NCC but did not affect the up-regulation of Kir4.1. n=4–6 in each group. Data are means±s.e. *P<0.01 versus sham control; #P<0.01 versus sham+TK; †P<0.01 versus siClC-K Con (ANOVA). In total protein lysates (lower), TK-mediated up-regulations of SPAK and NCC were also diminished by siClC-K administration. n=4–5 in each group. Data are means±s.e. *P<0.01 versus sham control; #P<0.01 versus sham+TK (ANOVA). TK-induced (Δ) increase in total NCC expression was compared between sham-si and siClC-K groups (Dashed lined area). n=4–5 in each group. Data are means±s.e. P<0.01 versus sham-si (t-test). Na-K-ATPase or GAPDH were used as loading controls of membrane protein or total protein, respectively. (b) Kir4.1 knockdown prevented TK-induced up-regulation of ClC-K and p-NCC in mDCT cell membrane (upper) and NCC in total lysate (lower). Na-K-ATPase or GAPDH were used as loading controls for membrane protein or total protein, respectively. n=4–6 in each group. *P<0.01 versus Control; #P<0.01 versus sham+TK (ANOVA).

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