Figure 4: Association of PKM2 with SNAP-23 in tumour cell exosomes. | Nature Communications

Figure 4: Association of PKM2 with SNAP-23 in tumour cell exosomes.

From: Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23

Figure 4

(a) WB analysis of isolated exosome fractions from Hela cells and A549 cells for PKM1, PKM2, p-PKM2 and SNAP-23 expression, 50 μg proteins of each sample were loaded. (b) Double staining of A549 cells with anti-PKM2 (green) and anti-SNAP-23 (red) antibodies. Arrows indicated the small punctate structures where SNAP-23 and PKM2 were co-localized. (c) Cross-IP and cross-blot of PKM2 and SNAP-23 in isolated exosome fraction from A549 cells using anti-SNAP-23 and anti-PKM2 antibodies, respectively. Immunoprecipitation using normal IgG served as controls.

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