Figure 5: PKM2 promotes the release of exosomes in tumour cells via phosphorylating SNAP-23.

The levels of phosphorylated or non-phosphorylated SNAP-23 in A549 cells assessed by SDS-PAGE or Phos-tag SDS-PAGE analysis, respectively. (a) A549 cells were treated with EGF or OA, or transfected with PKM2 siRNA or HA-PKM2-expressing plasmid. (b) A549 cells were treated with pTyr, FBP or serine. Data are presented as the mean±s.e.m. of three independent experiments. *P<0.05. **P<0.01 as determined by the one-way ANOVA test.