Figure 6: Direct phosphorylation of recombinant SNAP-23 (rSNAP-23) at Ser95 by recombinant PKM2 (rPKM2). | Nature Communications

Figure 6: Direct phosphorylation of recombinant SNAP-23 (rSNAP-23) at Ser95 by recombinant PKM2 (rPKM2).

From: Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23

Figure 6

(a) Direct phosphorylation of rSNAP-23 by rPKM2. The rSNAP-23 was incubated with or without PEP, rPKM2 or PEP plus rPKM2 at room temperature for 1 h. The reaction mixtures were then subjected to SDS-PAGE or Phos-tag SDS-PAGE analysis. SNAP-23 was detected by anti-SNAP-23 antibody in WB analysis. (b) Phosphorylated SNAP-23 by rPKM2 analysed by mass spectrometry (MS). Note that MS analysis of tryptic fragment of rSNAP-23 treated with PEP/rPKM2 matches to the peptide 92NFESGK97 of SNAP-23, suggesting that SNAP-23 Ser95 was phosphorylated.

Back to article page