Figure 1: Experimental set up for the detection of tumour heterogeneity in CRC.
From: DNA copy number changes define spatial patterns of heterogeneity in colorectal cancer
(a) Inter-patient and inter-tumour heterogeneity was investigated in 27 patients with 31 primary tumours and 66 metastases from different organs. Five patients are shown in detail (D4, D8, D27, D41 and D50). (b) Detailed information on the cohort studied. (c) Intra-tumour heterogeneity was investigated in a single stage II CRC tumour (tumour depicted in pink, colon in orange) which was isolated entirely and separated into 5 individual blocks. Each block was sectioned completely and each set of 15 sections was grouped together. Each section was divided into four compartments (A, B, C and D), resulting in a total of 68 samples used for massive parallel sequencing analysis. (d) Detailed information on the stage II CRC tumour studied. (e) H&E staining of one representative section depicting compartments A, B, C and D corresponding to the left and right lateral tumour regions, and the luminal and deep invasive front, respectively.
