Table 3 Primers used for generation of ADE1 constructs.
Name | Sequence |
|---|---|
FwdAde1SpeIWT | 5′GGactagtATGTCAATTACGAAGACTGAACTGG |
FwdAde1SpeI6AAA | 5′GGactagtATGAAAAAAAAAAAAAAAAAATCAATTACGAAGACTGAACTGG |
FwdAde1SpeI9AAA | 5′GGactagtATGAAAAAAAAAAAAAAAAAAAAAAAAAAATCAATTACGAAGACTGAACTGG |
FwdAde1SpeI12AAA | 5′GGactagtATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAATTACGAAGACTGAACTGG |
FwdAde1SpeI12AAG | 5′GGactagtATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGTCAATTACGAAGACTGAACTGG |
RevAde1ClaIFLAG | 5′GGatcgatTTACTTGTCGTCATCGTCCTTGTAGTCGTGAGACCATTTAGACCC |
FwdAdeIPromSacI | 5′GGgagctcACACGATAGCAAAGCAG |
RevAdeIPromXbaI | 5′ GGtctagaTATCGTTAATATTTCG |
