Figure 2: YAP1 signalling is modulated by fluid WSS and tumour location.
From: Fluid shear stress activates YAP1 to promote cancer cell motility
Gene expression was determined by Illumina HT-12 BeadChips in PC3 cells exposed to WSS or static conditions for 3 h. (a) Ingenuity pathway analysis reveals roles for WSS in a number of canonical pathways. (b) Hierarchical clustering of genes regulated by YAP1 segregates static and WSS-exposed cells (P<0.05). Colour key represents normalized expression within rows. (c) Elevated expression of YAP1/TAZ target genes was validated at several time points following initiation of WSS by qRT-PCR (n=3 independent experiments; Kruskal–Wallis one-way ANOVA, P<0.001, pairwise group differences were compared using the Dunn’s test, static versus WSS individual time point comparison, *P<0.05). (d) YAP1/TAZ and target genes expression were evaluated at static, WSS for 1 h and each recovery time point after WSS stopped. (e) Immunohistochemical staining of YAP1 in primary tumour and lymph node metastases (LN mets) of representative mouse implanted orthotopically with PC3 cells. Scale bar, 20 μm. (f) H-score of YAP1 protein expression in primary tumors and associated lymph nodes for three individual animals was calculated as the product of YAP1 intensity values (1–3) and per cent positive PC3 cells in the tumour (0–100), ranging from 0 to 300. Error bars represent±s.e.m.
