Figure 2: Differential gene expression of mESC transfected with either miR294 or let-7c. | Nature Communications

Figure 2: Differential gene expression of mESC transfected with either miR294 or let-7c.

From: The impact of microRNAs on transcriptional heterogeneity and gene co-expression across single embryonic stem cells

Figure 2

(a) Volcano plots showing expression fold change of mean across cells in each condition on x axis and -log10(FDR) on y axis. Individual cells within a condition were treated as repeats for differential expression analysis. Significantly differentially expressed miRNA targets (FDR <10%) for miR-294 and let-7c identified in previous population-based array experiments are highlighted as black triangles and P-values for the enrichment analysis is shown in upper left corner. miR-294 and let-7c targets are similarly highlighted in Dgcr8−/− versus WT mESC comparison. Conversely to let-7c, miR-294 is broadly expressed in mESC and its targets are significantly upregulated in miRNA-deficient Dgcr8−/− cells versus WT mESC. (b) Identification of discriminant processes between miR-294 and let-7c-transfected cells. Pathways are sorted according to their relevance by mean of the frequency they were selected by the RFE method to correctly assign a cell to its category. Only pathways selected at least in 10% of 1,000 trials are shown.

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