Figure 2: SPOC deficiency of kin4Δ cells is rescued by lack of FEAR.
From: Temporal and compartment-specific signals coordinate mitotic exit with spindle position
(a) Percentage of SPOC-deficient phenotypes observed in kar9Δ GFP-TUB1 cells with or without KIN4 and/or SPO12. Graph is an average of five independent experiments. Error bars show s.d. Per experiment, 100 cells were counted per strain. Asterisk indicates significant difference according to two-tailed Student’s t-test (P<0.05). (b) Duration of anaphase in kin4Δ kar9Δ and spo12Δ kin4Δ kar9Δ cells during spindle normal alignment and misalignment established by time-lapse microscopy of corresponding kar9Δ GFP-TUB1 cells. Duration of anaphase was calculated as the time elapsed from the start of fast spindle elongation (reference for metaphase-anaphase transition) until the spindle break down (reference for mitotic exit); n, number of cells analysed through time-lapse experiments with 1 min time resolution. Percentages show the prevalence of the indicated phenotypes. Note that kin4Δ kar9Δ cells exited mitosis within 20–23 min after metaphase–anaphase transition regardless of the spindle position. The anaphase took ∼23 min when the mitotic spindle was normally aligned in spo12Δ kin4Δ kar9Δ cells. In addition, 90% of the spo12Δ kin4Δ kar9Δ cells with misaligned spindles stayed arrested with intact spindles for >60 min. (c,d) Percentage of SPOC-deficient phenotypes in indicated cells types. Graph is an average of three independent experiments. Error bars show s.d. Per experiment, 100 cells were counted per strain. Asterisk indicates significant difference according to two-tailed Student’s t-test (P<0.05).
