Figure 3: FEAR activity during spindle misalignment.
From: Temporal and compartment-specific signals coordinate mitotic exit with spindle position
(a,b) Representative still images from the time-lapse series showing (a) Cdc14-GFP and (b) Sli15-GFP localization in the presence and absence of SPO12. The red dashed lines delineate Cdc14-GFP transiently released from nucleolus. The graphs show the percentage of cells with misaligned spindles with the indicated phenotypes. Per strain, 50 cells were analyzed by time lapse. Scale bars: 3 μm. The yellow dashed lines mark the cell boundary. Time point zero is the onset of anaphase (±1 min) based on the start of fast spindle elongation. Note that spo12Δ cells serve as a control where FEAR-released Cdc14 is blocked.
