Figure 2: SCIMP is recruited to TLR4 in macrophages on LPS activation. | Nature Communications

Figure 2: SCIMP is recruited to TLR4 in macrophages on LPS activation.

From: SCIMP is a transmembrane non-TIR TLR adaptor that promotes proinflammatory cytokine production from macrophages

Figure 2

(a) V5-labelled SCIMP was stably expressed in RAW264.7 macrophages, cells were treated with LPS, and at the indicated times, the V5 antibody was used for immunoprecipitation. Samples were probed for TLR4, pSFK(Y416), Lyn, Grb2 and V5-SCIMP. (b) Confocal imaging of LPS-treated (30 min) RAW264.7 cells transiently co-expressing TLR4-HA (green) and SCIMP-V5 (red), with phalloidin (blue). TLR4 and SCIMP are colocalized (white) on dorsal surface ruffles, as shown for separate channels in the Z projections. Data in a and b are representative of three independent experiments. The scale bar in b represents 10 μm.

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