Figure 4: SCIMP interacts directly with TLR4. | Nature Communications

Figure 4: SCIMP interacts directly with TLR4.

From: SCIMP is a transmembrane non-TIR TLR adaptor that promotes proinflammatory cytokine production from macrophages

Figure 4

(a) A schematic diagram depicting T1, T2 or full-length (FL) murine SCIMP with transmembrane domain (TMD), PRD, tyrosine residues 58, 96 and 120 (green bars) and N- and C-terminal amino acids. (b) Bacterially expressed GST tag alone, or GST-tagged SCIMP T1 or T2 was used to pull-down recombinant His-TLR4-TIR. (c) GST-SCIMP T1 or T2 fusion proteins were used in GST pull downs with extracts from LPS-activated RAW264.7 cells and probed for TLR4 and Lyn. (d) Titration of fluorescently labelled SCIMP-T1 with TLR4-TIR (left panel), with Kd (212 nM) determined by curve fit analysis (right panel). Data in b and c are representative of three independent experiments.

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