Figure 5: Fibre size is reduced in dKO mice.
From: Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy
(a) Representative image of fibre typing in WT quadriceps. Sections cut from the midbelly of 12 month old male quadriceps were stained with antibodies against MyHC Type 1 (green), 2A (blue) and 2B (red), while 2X fibres were left unstained. A488 conjugated wheat germ agglutinin was used to mark membranes to delineate the individual fibres (Green). Scale bar indicates 500 μm. (b) Fibre size frequency distribution for all quadriceps fibres (Approx 7,000 per quadriceps). The mean fibre size was reduced in dKO compared with mdx (1781±158 μm2 versus 1223±242 μm2, P<0.05). (c) Frequency distribution for all 2B fibres in the quadriceps. Mean 2B fibre size is reduced in dKO compared with WT (1950±213 μm2 versus 1670±267 μm2, P<0.05). (d) Individual fibre types presented as a percentage of total fibre number show no shift in fibre type proportion across all the genotype groups. Data shown as mean±s.e.m., WT n=6, mdx n=7, dKO n=6.
