Table 4 RT-qPCR primers and conditions.
From: Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy
Gene ID | Accession number | Forward oligo sequence (5′–3′) | Reverse oligo sequence (5′–3′) | Product size (bp) | Temp (°C) | Intron spanning |
|---|---|---|---|---|---|---|
Actb | NM_007393 | CCTCCCTGGAGAAGAGCTATG | TTACGGATGTCAACGTCACAC | 157 | 65 | Yes |
Rn18s | NR_003278 | GCAATTATTCCCCATGAACG | GGCCTCACTAAACCATCCAA | 124 | 65 | N/A |
Rer1 | NM_026395 | GCCTTGGGAATTTACCACCT | CTTCGAATGAAGGGACGAAA | 137 | 62 | Yes |
Rpl41 | NM_018860 | GCCATGAGAGCGAAGTGG | CTCCTGCAGGCGTCGTAG | 113 | 65 | Yes |
Pgc1α | NM_008904 | CAACAATGAGCCTGCGAAC | AGGGCAATCCGTCTTCATC | 116 | 65 | Yes |
