Table 1 PCR primers utilized to examine levels of BBB signature genes or transcripts altered in Glut1 DS.
Gene | Primer sequence |
---|---|
Glut1 F | 5′ CTT GCT TGT AGA GTG ACG ATC 3′ |
Glut1 R | 5′ CAG TGA TCC GAG CAC TGC TC 3′ |
Cldn3 F | 5′ GAT GGG AGC TGG GTT GTA CG 3′ |
Cldn3 R | 5′ GAG GAT CTT GGT GGG TGC AT 3′ |
Cldn5 F | 5′ GTT AAG GCA CGG GTA GCA CT 3′ |
Cldn5 R | 5′ TAC TTC TGT GAC ACC GGC AC 3′ |
Cldn12 F | 5′ GTC CTG TCC TTC CTG TGT GG 3′ |
Cldn12 R | 5′ TGA ATG TGA TCA GCC GCA GT 3′ |
Cdh5 F | 5′ ATT GGC CTG TGT TTT CGC AC 3′ |
Cdh5 R | 5′ CAC AGT GGG GTC ATC TGC AT 3′ |
Abcb1b F | 5′ GG GAG ATC CTC ACC AAG CG 3′ |
Abcb1b R | 5′ CCC ATC GCC CCT TA ACA CT 3′ |
Abcg2 F | 5′ TGG ACT CAA GCA CAG CGA AT 3′ |
Abcg2 R | 5′ CGG AAG CCA GTA AGG TGA GG 3′ |
Plvap F | 5′ CGT CAA GGC CAA GTC GCT 3′ |
Plvap R | 5′ CAG CAG GGT TGA CTA CAG GG 3′ |
β-actin F | 5′ TGT TAC CAA CTG GGA CGA CA 3′ |
β-actin R | 5′ GGG GTG TTG AAG GTC TCA AA 3′ |
Gapdh F | 5′ CGA CTT CAA CAG CAA CTC CCA CTC TTC C 3′ |
Gapdh R | 5′ TGG GTG GTC CAG GGT TTC TTA CTC CTT 3′ |
Vegfr2 F | 5′ CTA GCT GTC GCT CTG TGG TT 3′ |
Vegfr2 R | 5′ CTG TCC CCT GCA AGT AAT CTG A 3′ |