Figure 3: K16 and K6 are differentially regulated in TOC.
From: Rhomboid family member 2 regulates cytoskeletal stress-associated Keratin 16
(a) Western blot analysis of endogenous expression of K16 and K6 in CTRL and TOC keratinocytes. GAPDH was used as a loading control. (b) Confocal analysis performed on palm skin (positive control) and non-palmar TOC skin shows diminished K6 expression in the TOC epidermis. Scale bars, 20 μm. (c) Western blot analysis showing that IRHOM2 depletion by shRNA reduces the expression of K16 in CTRL and TOC keratinocytes, while it restores expression of K6 in TOC keratinocytes. GAPDH was used as a loading control. (d) Confocal analysis showing the reduction of K16 and K6 expression in Sh-iRHOM2 CTRL 3D human skin equivalents. Scale bars, 20 μm. (e) Confocal analysis showing the reduction of K16 expression but upregulation of K6 in Sh-iRHOM2 TOC 3D human skin equivalents. Scale bars, 20 μm.
