Figure 5: iRHOM2 regulates migration and proliferation.
From: Rhomboid family member 2 regulates cytoskeletal stress-associated Keratin 16
(a) Growth curves of cultured cells for Sh-Scr, Sh-IRHOM2 in CTRL and TOC keratinocytes showing slower growth rates for Sh-iRHOM2 cells in both cell lines (mean of three independent experiments). (b) Confocal analysis showing the reduction of Ki67 expression in Sh-IRHOM2 TOC 3D human skin equivalents. Scale bars, 20 μm. (c) Confocal analysis confirming the reduction of Ki67 expression in the forepaw epidermis of irhom2−/− mice. Scale bars, 20 μm. (d) Light-microscopy images of CTRL and TOC keratinocyte monolayers 0 and 24 h after scratch-wounding. Graph shows quantification of wound closure, mean of three experiments. Error bars denote s.d. *P<0.05 and ***P<0.001 (Student’s t-test). Scale bar, 20 μm. (e) Model of iRHOM2-mediated regulation of K16 in stressed epithelia.
