Figure 3: Spermatogenesis could not progress into meiosis in Bcas2F/−;Vasa-Cre testes.
From: BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis
(a) Western blotting analysis of MVH in control and Bcas2F/−;Vasa-Cre testes at P10, P12 and P15. α-tubulin was used as the loading control. (b) IF staining of MVH in control and Bcas2F/−;Vasa-Cre testes at P10, P12 and P15. The DNA was stained with Hoechst 33342. Scale bar, 50 μm. (c) Western blotting analyses of SCP3 and γH2AX in control and Bcas2F/−;Vasa-Cre testes at P10 and P12. α-tubulin was used as the loading control. (d–i) Hematoxylin and eosin (H&E) staining of control (d–f) and Bcas2F/−;Vasa-Cre testes (g–i) at P10, P12 and P15. Spermatogenic cells were shown in cross-sections of seminiferous tubules from control and Bcas2F/−;Vasa-Cre testes. Scale bar, 15 μm. Black arrows indicate the representative stages of the spermatocytes. L, leptotene; eP, early pachytene spermatocytes; Z, zygotene spermatocytes; P, pachytene spermatocytes; PL, pre-leptotene spermatocytes; red arrows, apoptotic cells. (j) Real-time RT-PCR analysis of marker gene expression in pre-meiotic testes from control and Bcas2F/−;Vasa-Cre males at P9 with Gapdh as the internal control. (*P<0.05; **P<0.01; ***P<0.001, n=5). Error bars represent s.e.m.
