Figure 5: Global analysis of Bcas2-mediated alternative splicing (AS).
From: BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis
(a) Scatter plot of significantly differentially expressed transcripts in Bcas2F/−;Vasa-Cre testes compared with the controls. Blue dots represent significantly down-regulated transcripts, while red dots show significantly up-regulated transcripts (P<0.05, fold-change of RPKM>1.5). Grey dots illustrated unchanged transcripts. (b) Real-time RT-PCR analysis of Pre-mRNA and mature (m) mRNA expression level of Tuba3a, Tuba3b and Tubb4b in control and Bcas2F/−;Vasa-Cre testes of P9 mice with Gapdh as the internal control (*P<0.05, n=5). Error bars represent s.e.m. The pre-mRNA primers were designed in one of the exons and the adjacent intron and the mature mRNA primers were designed to span an exon-exon junction. (c) Seven AS events significantly affected by depletion of BCAS2 in the testes at P9. The simple diagrams of seven AS events recognized by ASD software and splicing events affected by depletion of BCAS2 analysing the RNA-seq data using ASD software (P<0.05). (d) GO term enrichment analysis of genes with significantly affected AS events.
