Figure 2: 46B8 induces ADCC in vitro.
From: A broadly protective therapeutic antibody against influenza B virus with two mechanisms of action
(a) A549 cells infected with B/Brisbane/60/2008 were labelled with 46B8, Rituximab (negative control) or Cetuximab (positive control) prior to incubation with NK cells. NK cell activation (left panel) and target cell lysis (right panel) were presented as the frequency of CD107a+ cells and the percent of LDH release, respectively. (b) A549 cells infected with B/Brisbane/60/2008 were labelled with 46B8 or 46B8 N297G prior to incubation with NK cells. Target cell lysis was presented as the percent of LDH release. The assays were done in duplicate. Results are representative of three independent experiments. (a,b: mean and s.e.m.)
