Figure 8: The Sec3 Sso2-binding mutant is capable of tethering vesicles.
From: Sec3 promotes the initial binary t-SNARE complex assembly and membrane fusion
(a) Co-localization of Tom20-mCherry-SEC3 or Tom20-mCherry-sec3-mutS1 with Cit1-GFP, a marker for yeast mitochondria. Scale bar, 1 μm. (b) Ectopically targeted Tom20-mCherry-SEC3 and Tom20-mCherry-sec3-mutS1 were both able to recruit Sec6-GFP to mitochondria clusters. (c) Ectopically targeted Tom20-mCherry-SEC3 and Tom20-mCherry-sec3-mutS1 were both able to recruit Sec8-GFP to mitochondria clusters. (d) Yeast cells were fixed and spheroplasted for immunofluorescence staining of the Rab protein Sec4, a marker for post-Golgi secretory vesicles. Sec4 vesicles were recruited to mitochondria. (e) Quantification of yeast cells expressing Sec3 or sec3-mutS1 with mitochondria-exocyst co-localization and mitochondria-Sec4 co-localization. Three independent experiments were performed. In each experiment, 50 cells were counted. The percentage of cells with co-localization was plotted. Student’s t-test was used for statistical analyses. Error bars: s.e. No statistical significance was found comparing Sec3 and sec3-mutS1.
