Figure 2: Increased BBB permeability in cuprizone treated mice.
From: Dietary cholesterol promotes repair of demyelinated lesions in the adult brain
(a) Extravasation of Evans blue on sections of the corpus callosum. In control animals, Evans blue fluorescence is restricted to blood vessels but extravasates in mice on cuprizone (arrows) (scale, 50 μm). (b) BBB permeability was measured by Evans blue (EB) extravasation in brains of animals fed cuprizone (cup) for 5 weeks on normal chow or cholesterol supplemented chow, or in brains of animals with EAE 2d after the peak of clinical symptoms (n=3 animals). All treatment groups were normalized to untreated control animals (n=5) and compared by one way ANOVA (P<0.0001). Nutritional cholesterol did not influence BBB permeability. Bars represent mean±s.e.m. (c) Extravasation of bodipy-cholesterol. Maximum intensity projection of bodipy-cholesterol fluorescence in the corpus callosum (delineated by dashed lines) of mice that were kept on cuprizone for 5 weeks in comparison to untreated mice (control) (scale, 50 μm). (d) Quantification of bodipy-cholesterol extravasation after extraction. Data are expressed as fold changes±s.e.m. in cuprizone treated mice compared with untreated control animals (n=6 mice per group, unpaired Student’s t-test, P<0.0001).
