Figure 5: Single-molecule fluorescence imaging of DNA in sacrificial Si nanochannels. | Nature Communications

Figure 5: Single-molecule fluorescence imaging of DNA in sacrificial Si nanochannels.

From: Wafer-scale integration of sacrificial nanofluidic chips for detecting and manipulating single DNA molecules

Figure 5

(a) Optical image of nanofluidic regions with nanopillars and nanochannels (40 nm deep, 200 nm wide and 500 nm pitch). (b) Selected fluorescence images showing λ-DNA flowing through nanopillars and nanochannels corresponding to the optical images in a. Magenta and yellow dash lines indicate the pillar interface designed for straddling and the nanochannels entry point, respectively. Here frame 1 is defined the first frame the DNA molecule enters the imaged area. The DNA flowed from the bottom to the top. (c) The location-dependent DNA extension due to its hydrodynamic interactions with nanostructures, with the optical graph of the nanofluidic structures added as a location reference. Here the x axis origin is set as the nanochannel entry. Each black square dot represents the DNA extension in one frame, and the data point of frame 5 is labelled. The time interval between adjacent frames was ∼18 ms. The horizontal green dash-dot line indicates the estimated dyed lambda DNA extension when it is fully stretched. The scale bar in a is 10 μm.

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