Figure 5: Extracellular recombinant DEK does not enter the cell but restores NET formation in Dek-KO neutrophils.
From: DEK-targeting DNA aptamers as therapeutics for inflammatory arthritis
Dek-KO (n=3) BM neutrophils were treated with recombinant DEK prior to PMA stimulation. (a) Cells were fixed 2-3 h after PMA treatment, and then permeabilized and stained for DEK (red) and wheat germ agglutinin (WGA), a membrane marker. DEK is detected outside of the cell in the NETs (× 60 magnification, scale bar 20 μm, Nikon Confocal). (b) Cells were also stained for the NET marker myeloperoxidase (MPO, purple), DEK (red) and WGA (green). DEK is detected outside of the cell in NETs (× 60 magnification, scale bar 10 μm, Nikon Confocal). Images are representative of three different fields from at least three independent experiments.
