Figure 3: HA and CB inhibit Influenza-induced production of type I interferons in PBMC and mice.
From: Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement
(a–c) mRNA levels of IFN-α (a), IFN-β (b) and IFN-λ2/3 (c) from PBMC pre-incubated with histamine, and CB and stimulated overnight with Influenza, were measured by RT-qPCR and normalized to RPL13A. Data shown as the means of three independent experiments ±s.e.m. (d–f) 29S8 mice were infected with X31 (800 TCID50) and IFN-α (d), IFN-β (e) and IFN-λ2/3 (f) levels in BAL fluid were measured by ELISA (as described in methods). Data shown as the means ±s.e.m. ***P<0.0001, **P<0.001, *P<0.01 as measured by two-way ANOVA with Bonferroni post-tests.
