Figure 1: MACS1 is indispensable for morphogenesis of the respiratory apparatus in mouse larynx. | Nature Communications

Figure 1: MACS1 is indispensable for morphogenesis of the respiratory apparatus in mouse larynx.

From: Evolution of Shh endoderm enhancers during morphological transition from ventral lungs to dorsal gas bladder

Figure 1

Phenotypes of the respiratory apparatus in wild type (WT) (a–c and j–l), MFCS4−/− (d–f and m–o) and MACS1−/− (g–i and p–r) at E18.5. Deformed structures are encircled in yellow dashed lines. External appearance of the respiratory organs in WT (a), MFCS4−/− (d) and MACS1−/− (g). The thyroid cartilage is deformed in MACS1−/− (g). X-ray micro-CT sagittal image of WT (b), MFCS4−/− (e) and MACS1−/− (h). Morphological defects are apparent in the pharyngeal apparatus (soft palate and epiglottis) in MFCS4−/− (circled with yellow dashed line in e) and the laryngeal apparatus (arytenoids) in MACS1−/− (circled with yellow dashed line in h). 3D X-ray micro-CT images (c,f,i). WT develops arytenoids and epiglottis over the vocal folds (c). The epiglottis in MFCS4−/− is truncated (f), and the arytenoids and vocal folds in MACS1−/− (i) are deformed. X-ray micro-CT transverse images of the laryngeal apparatus (j–r). Arytenoids, epiglottis, vocal folds (circled with white dashed line in k) and thyroid cartilage are well developed in WT (j–l). The epiglottis in MFCS4−/− is truncated (m), and the thyroid cartilage and vocal fold are disrupted in MACS1−/− (q). Scale bars, 200 μm. An, arytenoids; As, arytenoid swelling; Ep, epiglottis; Lu, lung; Oe, oesophagus; Ph, pharynx; Sp, soft palate; Tc, thyroid cartilage; Tr, trachea; Trr, tracheal ring; Vf, vocal folds.

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