Figure 2: Interaction analysis of FT2α and FT2β with FD-like and 14-3-3 proteins.
From: Regulation of FT splicing by an endogenous cue in temperate grasses
(a) Interaction analysis of FT2α and FT2β with FD-like protein (FDL2) in yeast cells. Yeast cell growth on selective media without Leu, Trp, His and Ade (−LTHA) indicates positive interactions. (b) BiFC interaction assays of FT2α and FT2β with FDL2. Partial YFP fusion constructs were expressed transiently in N. benthamiana leaves. AHL22-RFP was used as a nuclear marker. Scale bar, 20 μm. (c) In vivo Co-IP assays of two FT2 splicing isoforms with FDL2. FLAG-tag FT2α or FLAG-tag FT2β were co-expressed with HA-tag FDL2 in N. benthamiana leaves. (d) Yeast two-hybrid analyses of interactions between FT2 isoforms and two 14-3-3 proteins (GF14b and GF14c). (e) BiFC interaction assays of FT2α and FT2β with 14-3-3 protein GF14b. Scale bar, 20 μm.
