Figure 1: F49A-FTP kills lytically infected cells because of their expression of US28.
From: Targeting the latent cytomegalovirus reservoir with an antiviral fusion toxin protein
Human foreskin fibroblast cells (HFFs) were infected with either HCMV Titan wild-type or HCMV Titan-ΔUS28 at an MOI of 0.1. Both viral isolates have a UL32-GFP tag, causing infected cells to appear green by fluorescence microscopy. Cultures were then either mock-treated with PBS or treated with 5 × 10−8 M F49A-FTP for 72 h and observed by fluorescence microscopy. (a) Representative images of the virally infected cultures with or without F49A-FTP. (b) A graphical representation of these data. Cell numbers were quantified by Hoechst staining cell nuclei, and the percentage of infected (green) cells is shown as a percentage of the control. White bars indicate 50 μm scale. Means and error bars (showing s.d.) were generated from three independent experiments. Statistical analyses were carried out using a paired two-tailed t-test and ***P=0.001 were considered significant.
