Figure 6: F49A-FTP kills naturally latently infected monocytes, reducing reactivation events.
From: Targeting the latent cytomegalovirus reservoir with an antiviral fusion toxin protein
1.2 × 108 blood monocytes were isolated from CMV-positive blood donors. Half of these cells were incubated with F49A-FTP for 72 h. Monocytes were then differentiated using granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) stimulation followed by LPS treatment. Monocyte-derived, mature dendritic cells (reactivated latent monocytes) were then co-cultured with fibroblasts for 3 weeks, and the numbers of reactivated IE foci were detected by immunofluorescent staining. Means and error bars (showing s.d.) were generated from three independent experiments. Statistical analyses were carried out using a paired two-tailed t-test and P values expressed as *P=0.05.
