Figure 3: Inside–out patch recordings from HEK293T cells expressing hexameric PANX1 concatemers with different numbers of intact C termini.
From: A quantized mechanism for activation of pannexin channels
(a) Diagram illustrates protocol for inside–out patch recording. (b) Steady-state inside–out patch recordings obtained at +70 mV from HEK293T cells expressing hexameric concatemers with 0 to 6 intact C termini. (c) Electron micrograph and class-averaged images of negatively stained single particles of 6(0CT), before (left, 315 of 13,240 particles were used for class average) and after TEVp cleavage (right, from 38 of 5,883 particles). Images of imposed six-fold symmetry are provided. Scale bar, 50 nm; class-averaged image, 35.7 × 35.7 nm2.
