Figure 3: Non-uniform temperature-specific changes in DENV2 and DENV1 at 37 and 40 °C by HDXMS.

Temperature-induced differences in deuterium exchange (t=1 min) in E-protein and M-protein from DENV2 (a,b, respectively) and DENV1 (e,f, respectively) between 28 and 37 °C (Orange line) and between 28 and 40 °C (Green line) are represented on a difference plot. Difference refers to the difference in average number of deuterons exchanged between two conditions. The difference plot displays the differences in exchange protein-wide where each dot represents a pepsin fragment peptide, listed from the N- to C-terminus. Y-axis-difference in deuterons, X-axis-pepsin fragment peptides. Differences in deuterium exchange above 0.5 D are considered significant (red dash line). Domain organisation of E-protein is indicated below the X-axis. S.e. for each peptide is shown as overlapping shaded regions along the X-axis and coloured according to the conditions in the difference plots. The individual s.e. in each condition is calculated as s.d.'s observed across all the HDXMS measurements (from at least three independent HDXMS experiments). The s.e. for a given peptide in the difference plot represents the sum of such single sigma s.d.'s of each of the two conditions being compared. Orthogonal views of the differences in deuterium exchange in E-protein peptides with temperature (37 °C in DENV2 (c,d) and 40 °C in DENV1 (g,h)) mapped onto the cryo-EM structure of E-M heterotetramer with one of the heterodimer rendered transparent for clarity. Regions with no peptide coverage are in grey.