Figure 4: Expansion of DENV2 and DENV1 captured by TR-FRET.
From: Conformational changes in intact dengue virus reveal serotype-specific expansion
(a) Representative fluorescence lifetime decay curve of AF488-TFP for dual labelled DENV2 at 25 (black) and 37 °C (grey), including the impulse response function (IRF) of the instrument. The lifetime traces are fitted with a two-component model. The average fluorescence lifetimes (<τf>) of AF488-TFP for donor-only labeled, <τf,SL> (grey triangles), and dual labelled, <τf,SL> (black circles), for DENV2 (NGC) (b) and DENV1 (PVP 159) (c). Error bars represent s.d.'s of six different experimental replicates in both DENV2 and DENV1 viruses. The temperature in these two cases was increased from 25 to 40 °C. The midpoint temperature of the transition was at ∼33 °C and greater than 37 °C for DENV2 and DENV1, respectively.
