Figure 5: Temperature expansion loci differ between DENV2 and DENV1.
From: Conformational changes in intact dengue virus reveal serotype-specific expansion
Isotopic mass spectral envelopes corresponding to representative homologous peptides of equivalent lengths of DENV2 (a) and DENV1 (b) E-proteins spanning the two, three and five-fold interaction interfaces after deuterium exchange of t=0 and 1 min at 28, 37 and 40 °C. Dashed lines represent centroid of mass envelope. Differences in sequence between DENV2 and DENV1 are indicated in black. (c) Temperature-specific changes at 37 °C mapped onto unexpanded DENV2 structure (PDB ID: 3J27). (d) Temperature-specific changes at 40 °C mapped onto unexpanded DENV1 structure (PDB ID: 4CCT). Regions with no peptide coverage are grey.
