Figure 2: OncoPPi network architecture.

(a) A connectivity map of the OncoPPi network involving 83 lung cancer-associated proteins linked via 397 interactions (Supplementary Cytoscape file available for visualization and detailed analysis, see Supplementary Data 3). Major hubs highlighted in green. PPIs with mutual exclusivity of genomic alterations in LUAD are indicated with blue lines. (b) Analysis of network topology, including degree and BC reveals major PPI hubs. (c) Heatmap showing GO annotations of cellular localization for OncoPPi network genes. (d) Bar graph showing the number of OncoPPi PPIs supported by predicted domain–domain interactions. Domain–domain pairs in OncoPPi using Pfam domain annotation are listed on the x axis, the number of PPIs in OncoPPi on the y axis. Examples of co-crystallized CDK4/CyclinD, ARNT/HIF1α, and a homology model of MST1/RASSF1 constructed with the Swiss-Model server (swissmodel.expasy.org) based on MST1/RASSF5 crystal structure are shown to illustrate the interactions between different structural domains. (e) Venn diagram showing the distribution of PPIs in the OncoPPi network supported by cellular co-localization (Co-loc) data and/or structural domain–domain interactions (DDI). See also Supplementary Fig. 3.