Figure 3: Disruption of FAK impairs adipose tissue expansion with caloric excess.

(a) Photograph of 24-week-old littermate control and aP2FAK^−/− mouse fed chow or HFD for 16 weeks, and littermate control aP2FAK^+/+ db/db and aP2FAK^−/− db/db mouse. (b–e) Body weight of male (n=12) (b) or female (n=8) (c) mice fed HFD starting at 8 weeks of age or mice on db/db (n=10) (d) or ob/ob (n=8) (e) genetic background. (f) Body composition expressed as per cent total body weight in 20- to 24-week-old male (green) and female (purple) mice fed HFD diet for 12–16 weeks (n=6). (g–i) Perigonadal WAT sections stained with haematoxylin and eosin (H&E) (scale bar, 100 μm; arrows indicate macrophage crown-like structures) with adipocyte size distribution (g), average adipocyte diameter (h) and calculated total adipocyte number (i) from 20- to 24-week-old mice fed HFD for 12–16 weeks (n=4 mice). Data are mean±s.e.m. *P<0.05 and **P<0.01 by Student’s t-test between means.