Figure 6: Impaired adipose tissue expansion with disruption of FAK cannot be overcome by PPARγ agonist.

(a–c) Relative expression of adipogenic genes from perigonadal WAT of aP2FAK^−/− versus control 20- to 24-week-old mice fed chow diet (n=7) (a) or HFD (n=5) (b) and in 3T3-L1 cells day 3 after FAK versus control scramble siRNA treatment (n=6) (c). (d) Oil red O staining of differentiated 3T3-L1 adipocytes day 7 following siRNA knockdown of FAK (scale bar, 100 μm). (e) Body weight in 20- to 24-week-old control or aP2FAK^−/− mice fed HFD with rosiglitazone for 12–16 weeks (n=7). (f) Body composition expressed as percent total body weight (n=10). (g) ITT in 20- to 24-week-old mice fed HFD with rosiglitazone for 12–16 weeks (n=5). Data are mean±s.e.m. *P<0.05 by Student’s t-test.