Figure 5: Tuning circuit output.

Logic circuit diagram, seesaw circuit diagram and experimental data of a two-layer logic circuit with (a) two upstream OR gates connected to a downstream AND gate and (b) two upstream AND gates connected to a downstream OR gate. Nominal concentrations shown in grey and black indicate adjustments made in a previous step and in this step, respectively. Small insets of experimental data show the circuit behaviours before adjustments. 1 × =100 nM.