Figure 2: Snail suppresses aerobic glycolysis in cancer cells. | Nature Communications

Figure 2: Snail suppresses aerobic glycolysis in cancer cells.

From: Snail reprograms glucose metabolism by repressing phosphofructokinase PFKP allowing cancer cell survival under metabolic stress

Figure 2

(a,b) Metabolic profile analysis following knockdown of Snail in MDA-MB-231 cells. OPLS-DA score plot (R2X=0.888, R2Y=0.992, Q2=0.945) (a) and loading column plot (b) of metabolite concentrations through targeted profiling derived from 1H NMR spectra of MDA-MB-231 cell extracts expressing control shRNA (shControl) and Snail shRNA (shSnail). (c) A diagram and quantification of the changes in intracellular metabolites in MDA-MB-231 cells. *,** and *** indicate P<0.05, P<0.01 and P<0.001 calculated from the Mann–Whitney U test. NAD+, nicotinamide adenine dinucleotide (oxidized form); O-PC, O-phosphocholine. The x and y axis represent experimental groups (blue bars, shControl; red bars, shSnail) and metabolite concentration (μM), respectively. (d) Quantification of intracellular lactate following knockdown of Snail in MCF-7 and MDA-MB-231 cells. ** P<0.01 compared with the control by Student’s t-test. (e) The relative abundance of 13C-lactate from MDA-MB-231 cell extracts was quantified from 1D 1H{13C} HSQC peak intensity. Data are means±s.d. from n=5 independent experiments. ** P<0.01 compared with the control by Student’s t-test.

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