Figure 2: p190-B regulates TGF-β signaling following serial transplantation. | Nature Communications

Figure 2: p190-B regulates TGF-β signaling following serial transplantation.

From: p190-B RhoGAP and intracellular cytokine signals balance hematopoietic stem and progenitor cell self-renewal and differentiation

Figure 2

(a–c) LSK-SLAM cells were isolated from three independent 2T mice per group, three independent times, and used for microarray analyses. (a) Heat map of genes differentially expressed, top candidate genes based on Student’s t-test values. (b) Unbiased gene set enrichment analysis of differentially expressed gene, FDR<0.1. (c) Bar graphs show the gene ontology results for molecular and biological processes with the indicated numbers of genes that were different in 2T-p190-B−/− HSC, of top differentially expressed candidate genes, analysed in TopGene Suite, P<0.0001. (d) CFU after in vitro culture. LSK-SLAM cells from 2T-WT mice were cultured with SCF+TPO for 4 days in the presence of inhibitors of various signaling pathways and cells from 2T-p190-B−/− were treated with TGFBRI inhibitor 1;, and then plated in CFU assay without inhibitors to assess progenitor production. Data represented as fold change in CFUs of cultured cells compared with non-treated 2T-WT cells from 3 independent experiments (mean±s.e.m.). *P<0.05, two-tailed unpaired t-test. (e) mRNA expression analyses by qPCR of TGF-β signaling target genes—tgif2 and smurf2 in LSK cells isolated from control, 2T-WT and 2T-p190B−/− mice. Data are normalized to β actin and presented as fold changes relative to non-transplanted cells. (mean±s.e.m.; n=3–5 from three independent experiments). *P<0.05, two-tailed unpaired t-test.

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