Figure 3: Changes in contractility are paralleled by actin and vinculin remodelling.
From: Optogenetic control of cellular forces and mechanotransduction
(a,c) iRFP signal of MDCK cells expressing optoGEF-RhoA, lifeact-iRFP and either CIBN-GFP-CAAX (a) or mito-CIBN-GFP (c) before, during and after 11 min of illumination with blue light. (b,d) Quantification of lifeact fluorescence intensity at the basal confocal plane over time normalized by the mean fluorescence intensity before the start of activation (in b, n=23 cells; in d, n=22 cells). (e) iRFP signal of MDCK cells expressing optoGEF-RhoA, vinculin-iRFP and mito-CIBN-GFP before, during and after 11 min of illumination with blue light. (f) Quantification of focal adhesion fluorescence intensity at the basal plane normalized by the mean value before activation (n=9). Arrows indicate the time points when largest structural changes occur. Shaded areas are s.d. Scale bars, 20 μm.
