Figure 6: Determination of the stoichiometry of BV binding to Syn BVR. | Nature Communications

Figure 6: Determination of the stoichiometry of BV binding to Syn BVR.

From: A substrate-bound structure of cyanobacterial biliverdin reductase identifies stacked substrates as critical for activity

Figure 6

(a) BV titration of Syn BVR (10 μM), as determined from the difference absorption spectra acquired in the presence of NADP+. Arrows indicate trends of spectral changes. (b) Titration curve of Syn BVR observed at 740 nm. (c) Titration curve of the Syn BVR R185A mutant. Measurements were performed in triplicate.

Back to article page