Figure 3: The I. scapularis IMD pathway responds to A. phagocytophilum infection.
From: Infection-derived lipids elicit an immune deficiency circuit in arthropods
(a–d) ISE6 (1 × 105) cells were infected with A. phagocytophilum (MOI 50) following targeted gene silencing of (a) xiap, (b) bendless/uev1a, (c) relish and (d) caspar. Gene silencing and A. phagocytophilum load (16 s rDNA) were measured by quantitative reverse transcriptase–PCR at 18 h post-infection in ISE6 cells. Replicates of 5 were expressed as means±s.e.m. All experiments shown are representative of five biological replicates with two technical replicates each. Student’s t test. *P<0.05. scRNA, scrambled RNA; siRNA, small interference RNA. See also Supplementary Table 2.
