Figure 4: Cortical noise injection before and during tone presentation disrupts behavioural performance.
From: Dynamics of auditory cortical activity during behavioural engagement and auditory perception
(a) Top: expression of the ChETA-EYFP construct was induced by injecting a virus bilaterally in the auditory cortex of trained rats. Bottom: appropriate expression was confirmed by immunohistochemistry for the GFP protein (green). Scale bar, 2 mm. (b) Top: raster plot for an example neuron recorded during optical stimulation (blue lines) of the auditory cortex in a freely behaving rat. Bottom, z-score PSTH for the example recording above shows that light stimulation can drive activity in the auditory cortex. (c) Top: schematic of the stimulation protocol: a train of 5 ms blue light pulses was delivered bilaterally or unilaterally at 20–30 Hz in the auditory cortex during the behavioural trial. Self-initiation triggered the light train, which lasted until tone offset. The ‘light ON’ trials (blue) were interleaved at a 1:5 ratio with ‘light OFF’ trials (black). Bottom: compared with control values, hit rate decreased during full-trial activation of the auditory cortex (blue line). (d) Top: schematic of the stimulation protocols. Bottom: hit rate performance. Error bars are s.e.m.
