Figure 3: Molecular regulatory mechanisms of rs1800734 on DCLK3. | Nature Communications

Figure 3: Molecular regulatory mechanisms of rs1800734 on DCLK3.

From: The non-coding variant rs1800734 enhances DCLK3 expression through long-range interaction and promotes colorectal cancer progression

Figure 3

(a) Isogenic cell lines possessing homozygous mutations (G/G and A/A) at rs1800734 were generated and (b) confirmed, and (c) the expression of DCLK3 was elevated in the A/A cell line (data are represented as mean and error bars indicate s.d., n=4 biological replicates per cell line, P-values: Student’s t-test). (d) 4C-seq identified chromatin interactions between rs1800734 and the DCLK3 region. (e) The A/A cell line showed stronger chromatin interaction than other cell lines (data are represented as mean and error bars indicate s.d., n=2 biological replicates per cell line). (f) The enhanced chromatin interaction results in increased chromatin accessibility at 3′-UTR region of DCLK3 in the A/A line.

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