Figure 1: Identification of lncRNAs capable of activating AKT by SAM library screen along with an AKT reporter. | Nature Communications

Figure 1: Identification of lncRNAs capable of activating AKT by SAM library screen along with an AKT reporter.

From: LncRNA AK023948 is a positive regulator of AKT

Figure 1

(a) Schematic description of principle for the AKT reporter used to screen the SAM library. SAM gRNAs that can activate AKT activity are enriched by puromycin selection. (b) Detection of AKT activity for cells before selection (BS) and cells after selection (AS) by western blot. (c) AK023948 SAM gRNA increases the pAKT level. A mixed pool of five SAM gRNAs against AK023948 were introduced into MCF-7 cells carrying dCas9-VP64 and pMS2-p65-HSF1 by infection and cellular extract was prepared for western blot 2 days after infection.

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