Figure 3: DHX9 is an AK023948-binding partner and is involved in regulation of AKT activity. | Nature Communications

Figure 3: DHX9 is an AK023948-binding partner and is involved in regulation of AKT activity.

From: LncRNA AK023948 is a positive regulator of AKT

Figure 3

(a) RNA precipitation using the biotin-labelled AK023948 probe, followed by PAGE and silver staining. Red star indicates a unique band bound to AK023948. Mass spectrometry analysis suggested DHX9 as a candidate. (b) Confirmation of the interaction between AK023948 and DHX9 by RNA precipitation and western blot. (c) Confirmation of the interaction between AK023948 and DHX9 by RNA immunoprecipitation using DHX9 antibody. (d) While DHX9 siRNA suppresses, ectopic expression of DHX9 increases the pAKT level. DHX9 siRNAs or DHX9 expression vector was introduced into MCF-7 cells by transfection, and cellular extract was prepared for western blot 48 h after transfection. Values in c are s.e.m. (n=3). **P<0.01 by two-tailed Student’s t-test.

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